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prolong gold anti-fade reagent containing dapi  (Thermo Fisher)


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    Thermo Fisher prolong gold anti-fade reagent containing dapi
    Prolong Gold Anti Fade Reagent Containing Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong%C2%AE+gold+anti-fade+reagent+with+dapi/prolong+diamond+antifade+mountant/pmc12163151-65-21-22
    Average 90 stars, based on 1 article reviews
    prolong gold anti-fade reagent containing dapi - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Laser-Scanning Microscopy:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Expressing:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Negative Control:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Immunofluorescence:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Staining:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Western Blot:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Membrane:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Control:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Immunoprecipitation:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Positive Control:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    In Vitro:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).

    Cell Culture:

    Article Title: SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles
    Article Snippet: Sections were mounted with a drop of Prolong® Gold anti-fade reagent with DAPI (ThermoFisher) and were imaged using an inverted Leica SP5 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).

    Article Title: Deacetylisovaltratum disrupts microtubule dynamics and causes G 2 /M-phase arrest in human gastric cancer cells in vitro.
    Article Snippet: The slides were sealed with cover glasses using the ProLong® Gold anti-fade reagent with DAPI (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) and immediately observed by confocal microscopy (Leica SP8, Mannheim, Germany).



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    prolong gold anti fade reagent with dapi  (Fisher Scientific)
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    Fisher Scientific prolong gold anti fade reagent with dapi
    Prolong Gold Anti Fade Reagent With Dapi, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GelMa/SCS-MN promotes re-epithelialization and revascularization in diabetic wounds. (a) Photographs of wounds of diabetic mice at different time after treatments. (b) Quantitative analysis of the relative residual wound areas at different times (n = 6). (c) Sagittal sections of day 3 and day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5, green) and <t>DAPI</t> (blue). Scale bar, 200 μm. (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), Itga5 (green) and labeled with 5-ethynyl-2′-deoxyuridine (EdU, gray) (proliferation), cell nucleus for DAPI (blue). Scale bar, 100 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 5). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 5). (g) Sagittal sections of day7 wounds tissue immunolabeled for Endomucin (Emcn, red) and EdU (green), cell nucleus for DAPI (blue). Scale bar, 200 μm. (h) Quantitative statistics of the Emcn + vascular gap in wound (n = 5). (i) Representative H&E staining of diabetic wound tissue on day7 and day 14. The green line indicates epidermal gap. Scale bar, 200 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day 7 and day 14 according to HE staining images (n = 3). (l) Sagittal sections of day14 wounds tissue immunolabeled for Emcn (red), cell nucleus for DAPI (blue). Scale bar, 200 μm. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey's multiple comparison test for (f), (h) and (j), two-way ANOVA with Sidak's multiple comparison test for (b), (e), and (k). P value: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Prolong Gold Anti Fade Reagent With Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prolong gold anti-fade reagent containing dapi  (Thermo Fisher)
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    GelMa/SCS-MN promotes re-epithelialization and revascularization in diabetic wounds. (a) Photographs of wounds of diabetic mice at different time after treatments. (b) Quantitative analysis of the relative residual wound areas at different times (n = 6). (c) Sagittal sections of day 3 and day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5, green) and <t>DAPI</t> (blue). Scale bar, 200 μm. (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), Itga5 (green) and labeled with 5-ethynyl-2′-deoxyuridine (EdU, gray) (proliferation), cell nucleus for DAPI (blue). Scale bar, 100 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 5). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 5). (g) Sagittal sections of day7 wounds tissue immunolabeled for Endomucin (Emcn, red) and EdU (green), cell nucleus for DAPI (blue). Scale bar, 200 μm. (h) Quantitative statistics of the Emcn + vascular gap in wound (n = 5). (i) Representative H&E staining of diabetic wound tissue on day7 and day 14. The green line indicates epidermal gap. Scale bar, 200 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day 7 and day 14 according to HE staining images (n = 3). (l) Sagittal sections of day14 wounds tissue immunolabeled for Emcn (red), cell nucleus for DAPI (blue). Scale bar, 200 μm. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey's multiple comparison test for (f), (h) and (j), two-way ANOVA with Sidak's multiple comparison test for (b), (e), and (k). P value: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
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    Image Search Results


    GelMa/SCS-MN promotes re-epithelialization and revascularization in diabetic wounds. (a) Photographs of wounds of diabetic mice at different time after treatments. (b) Quantitative analysis of the relative residual wound areas at different times (n = 6). (c) Sagittal sections of day 3 and day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5, green) and DAPI (blue). Scale bar, 200 μm. (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), Itga5 (green) and labeled with 5-ethynyl-2′-deoxyuridine (EdU, gray) (proliferation), cell nucleus for DAPI (blue). Scale bar, 100 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 5). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 5). (g) Sagittal sections of day7 wounds tissue immunolabeled for Endomucin (Emcn, red) and EdU (green), cell nucleus for DAPI (blue). Scale bar, 200 μm. (h) Quantitative statistics of the Emcn + vascular gap in wound (n = 5). (i) Representative H&E staining of diabetic wound tissue on day7 and day 14. The green line indicates epidermal gap. Scale bar, 200 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day 7 and day 14 according to HE staining images (n = 3). (l) Sagittal sections of day14 wounds tissue immunolabeled for Emcn (red), cell nucleus for DAPI (blue). Scale bar, 200 μm. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey's multiple comparison test for (f), (h) and (j), two-way ANOVA with Sidak's multiple comparison test for (b), (e), and (k). P value: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Sulfated polysaccharide facilitates macrophage-Treg crosstalk to mitigate chronic inflammation in diabetic wound healing

    doi: 10.1016/j.bioactmat.2025.09.044

    Figure Lengend Snippet: GelMa/SCS-MN promotes re-epithelialization and revascularization in diabetic wounds. (a) Photographs of wounds of diabetic mice at different time after treatments. (b) Quantitative analysis of the relative residual wound areas at different times (n = 6). (c) Sagittal sections of day 3 and day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5, green) and DAPI (blue). Scale bar, 200 μm. (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), Itga5 (green) and labeled with 5-ethynyl-2′-deoxyuridine (EdU, gray) (proliferation), cell nucleus for DAPI (blue). Scale bar, 100 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 5). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 5). (g) Sagittal sections of day7 wounds tissue immunolabeled for Endomucin (Emcn, red) and EdU (green), cell nucleus for DAPI (blue). Scale bar, 200 μm. (h) Quantitative statistics of the Emcn + vascular gap in wound (n = 5). (i) Representative H&E staining of diabetic wound tissue on day7 and day 14. The green line indicates epidermal gap. Scale bar, 200 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day 7 and day 14 according to HE staining images (n = 3). (l) Sagittal sections of day14 wounds tissue immunolabeled for Emcn (red), cell nucleus for DAPI (blue). Scale bar, 200 μm. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey's multiple comparison test for (f), (h) and (j), two-way ANOVA with Sidak's multiple comparison test for (b), (e), and (k). P value: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: The specimens were sectioned to a thickness of 10 μm, washed three times with TBST, and mounted using ProLong gold anti-fade reagent with DAPI (Cell Signaling Technology).

    Techniques: Immunolabeling, Labeling, Migration, Staining, Comparison

    Lacking Tregs impairs SCS-mediated diabetic wound healing. (a) Schematic illustrating experiment depleting Tregs. (b) Flow cytometric quantification of Tregs (Foxp3 + ) in the diabetic skin wound after anti-CD25 mAb injection (n = 6). (c) Representative wound images of diabetic mice after the indicated treatments and the corresponding time-course quantification of relative residual wound area (n = 8). (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5) (green), EdU (gray) and DAPI (blue). Scale bar, 200 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 8). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 12). (g) Representative flow cytometric plots of CD31 + Emcn + cells expression in diabetic wound tissue on day7 post-wounding and corresponding quantitative results (n = 6). (h) Representative immunostainings of CD31 (red), Emcn (green), EdU (gray) and DAPI (blue) in diabetic wound tissue on day7 post-wounding. Scale bar, 200 μm. (i) Representative H&E staining of diabetic wound tissue on day7 and day14. Scale bar, 500 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day14 according to H&E staining images (n = 3). Data are presented as mean ± SD and statistical significance was analyzed via two-way ANOVA with Sidak's multiple comparison test for (c), unpaired two-tailed Student's t -test for (b), (e), (f), (g), (j) and (k). P value: ∗P < 0.05, ∗∗P < 0.01.

    Journal: Bioactive Materials

    Article Title: Sulfated polysaccharide facilitates macrophage-Treg crosstalk to mitigate chronic inflammation in diabetic wound healing

    doi: 10.1016/j.bioactmat.2025.09.044

    Figure Lengend Snippet: Lacking Tregs impairs SCS-mediated diabetic wound healing. (a) Schematic illustrating experiment depleting Tregs. (b) Flow cytometric quantification of Tregs (Foxp3 + ) in the diabetic skin wound after anti-CD25 mAb injection (n = 6). (c) Representative wound images of diabetic mice after the indicated treatments and the corresponding time-course quantification of relative residual wound area (n = 8). (d) Sagittal sections of day 7 wound tissue immunolabeled for cytokeratin (red), integrin a5 (Itga5) (green), EdU (gray) and DAPI (blue). Scale bar, 200 μm. (e) Quantitative statistics of the migration distance of epidermal tongue from wound edge (n = 8). (f) Quantitative statistics of the number of EdU + epidermal cells in each visual field (n = 12). (g) Representative flow cytometric plots of CD31 + Emcn + cells expression in diabetic wound tissue on day7 post-wounding and corresponding quantitative results (n = 6). (h) Representative immunostainings of CD31 (red), Emcn (green), EdU (gray) and DAPI (blue) in diabetic wound tissue on day7 post-wounding. Scale bar, 200 μm. (i) Representative H&E staining of diabetic wound tissue on day7 and day14. Scale bar, 500 μm. (j–k) Quantification of (j) epidermis gap on day 7 and (k) scar distance on day14 according to H&E staining images (n = 3). Data are presented as mean ± SD and statistical significance was analyzed via two-way ANOVA with Sidak's multiple comparison test for (c), unpaired two-tailed Student's t -test for (b), (e), (f), (g), (j) and (k). P value: ∗P < 0.05, ∗∗P < 0.01.

    Article Snippet: The specimens were sectioned to a thickness of 10 μm, washed three times with TBST, and mounted using ProLong gold anti-fade reagent with DAPI (Cell Signaling Technology).

    Techniques: Injection, Immunolabeling, Migration, Expressing, Staining, Comparison, Two Tailed Test